| | Category | BO | L09 | The Development of Fluorescently Tagged Organelles in Arabidopsis |
| | thaliana |
| | Abstract | Currently, a set of tools is lacking for the stable localization of proteins and |
| | protein complexes in Arabidopsis thaliana. The development of a set of |
| | tools available for mutant lines, such as Non-race specific disease |
| | resistance 1 (ndr-1), and wild-type Columbia (Col-0), would make protein |
| | co-localization experiments more sensitive. This would lead, for example, |
| | to a better understanding of the protein to protein interactions inside the |
| | plant cell associated with defense-related signaling, such as the protein |
| | trafficking of Actin de-polymerization 4 (adf-4). In particular, organelles |
| | such as peroxisomes, plastids, vacuoles, endoplasmic reticulum, and the |
| | Golgi have been tagged with three different fluorescent proteins (FP) |
| | (green, yellow, and cyan) in three different plasmids, Col-0, ndr1, and adf- |
| | 4, for kanamycin and basta selection for flexibility purposes. This set of |
| | tools should be an asset to the plant community for protein co-localization |
| | studies in plant defense. |
| | Bibliography | Nelson, B.K., Cai, X., Nebenführ, A. (2007) A multicolored set of in vivo |
| | organelle markers for co-localization studies in Arabidopsis and other |
| | plants. Plant J., 51, 1126-1136. Century, K.S., Holub E.B., Staskawicz B.J. |
| | (1995) NDR1, a locus of Arabidopsis thaliana that is required for disease |
| | resistance to both a bacterial and a fungal pathogen. Natl. Acad. Sci. USA, |
| | 92, 6597-6601. |